Training on Immuno Biochemical Techniques on Department of Veterinary Biochemistry of West Bengal University Animal & Fishery Science PROJECT Detection of MONODON BACULO VIRUS by PCR method WORK ON MOLECULAR BIOLOGY:- 1. Preparation of competent cells (E. coli BL21)&transformation of these cells with plasmid DNA 2. DNA ligation 3. Bacterial genomic DNA isolation 4. Expression of recombinant protein 5. Bacterial conjugation 6. Restriction digestion 7. Isolation of RNA from prokaryotic source 8. PCR 9. SNP using specific primers by PCR &to establish genetic variation 10. Bacterial gene expression 11. SDS PAGE 12. Western blotting 13. RAPD 14. Genomic DNA isolation from E.coli IMMUNOLOGY:- 1. Ouchterlony double diffusion method 2. Rocket electrophoresis 3. Immuno electrophoresis 4. ELISA 5. Coomb’s test MICROBIAL BIOTECHNOLOGY:- 1. Determination of Thermal Death Point 2. Antibiotic assay of tetracycline by agar cup plate method 3. Characterization of protein in spectroscopic analysis, isolation from E. coli by alkaline phosphatase 4. Preparation of Et0H in laboratory scale 5. Isolation of heavy metal resistant bacteria from soil MODEL ORGANISM:- 1. Breeding experiment of zebra fish 2. Study of polytene chromosome in Drosophila melanoglaster 3. Permanent whole mount preparation of head of Drosophila melanoglaster 4. Study of genectic basis of segmentation by making cuticular preparation of wild type embryo of Drosophila melanoglaster 5. Identification of genes in Drosophila melanoglaster by pP element insertional mutagenesis 6. Preparation of salivary gland chromosome of Drosophila melanoglaster to study chromosome inversion 7. Studies of pattern formation hedgehog gene in wing imaginal disc of Drosophila melanoglaster by repoter gene LacZ expression GENETIC TOXICITY TESTING:- 1. Genotoxicity test on mice 2. Genotoxicology study in plant root tip(onion) CYTOGENETICS:- Chromosome analysis & karyotype reporting